Journal:

Article Title: Beta-3 adrenergic stimulation of L -Type Ca 2+ channels in rat portal vein myocytes

doi: 10.1038/sj.bjp.0703187

Figure Lengend Snippet: Expression of β-adrenoceptor mRNA in rat portal vein and detrusor smooth muscles. Amplified cDNA fragments corresponding to β1-, β2- and β3-adrenoceptors (AR) were separated on a 2% agarose gel and visualized by staining with ethidium bromide. HinfI: molecular size standards in base pairs (bp). For RNA purification and PCR conditions, see Methods.

Article Snippet: Molecular-weight marker, HinfI, was from Promega (Charbonnières, France).

Techniques: Expressing, Muscles, Amplification, Agarose Gel Electrophoresis, Staining, Purification

Journal: Journal of Bacteriology

Article Title: The Master Regulators of the Fla1 and Fla2 Flagella of Rhodobacter sphaeroides Control the Expression of Their Cognate CheY Proteins

doi: 10.1128/JB.00670-16

Figure Lengend Snippet: Determination of the presence of CheY3 in different strains by immunoblotting and plot profile of the primer extension experiment for cheOp2. (A) Total cell extracts obtained from cell cultures grown in Sistrom's minimal medium and harvested at an OD600 of 0.6 were tested as described in Materials and Methods. Anti-CheY3 antibodies were used at 1:3,000. WT, WS8N cell extract. Each lane is labeled according to the relevant mutation present in the strain. Other labels are the same as described in the legend for Fig. 4A. (B) Total RNA was isolated from cell cultures of WS8N and SP13 (ΔfleQ::kan) strains grown in Sistrom's minimal medium. After hybridization of the RNA samples with the 5′-end-labeled oligonucleotide, cDNA synthesis was performed using AMV reverse transcriptase. The reactions were subjected to gel electrophoresis, and the images were visualized using a Typhoon scanner (GE Healthcare Life Science) and quantified using ImageJ. The plot profiles initiate immediately below the largest transcript detected (position −155 from the translational start site of RSWS8N_13355), denoted by an open circle. The transcripts that originated from the σ70 and σ28 promoters are indicated by vertical arrows. The asterisks correspond to the migration of the 151-, 140-, 118-, 100-, and 82-bp (from left to right) DNA fragments of PhiX174 that were obtained after digestion with HinfI (Promega) and 5′ end labeled.

Article Snippet: As a molecular marker, we used DNA from PhiX174 digested with HinfI (Promega) and 5′ end labeled with [γ- 32 P]ATP.

Techniques: Western Blot, Labeling, Mutagenesis, Isolation, Hybridization, cDNA Synthesis, Reverse Transcription, Nucleic Acid Electrophoresis, Migration